Coding

Part:BBa_K4632015

Designed by: Haolong Lai   Group: iGEM23_SCAU-China   (2023-10-09)


LasI

Coding region for lasI protein, which produces the chemical signal AI-1.

We improved this part (BBa_C0078[1]) by optimizing the codons for Escherichia coli expression. Codon optimization was done with the help of GUANGZHOU IGE BIOTECHNOLOGY LTD. We successfully characterized its performance in E. coli TOP10. For more detailed characterization results, please refer to our composite_part-Part:BBa_K4632024[2].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 602
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 602
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None